Jeff Chalmers Laboratory for Cell Analysis and Separation
Jeff Chalmers Laboratory for Cell Analysis and Separation
Link List
About Us
Mankind is currently experiencing a revolution in our understanding of biological processes, from those occurring in “simple” bacteria to those occurring in human beings. Like other scientific revolutions of the past, rapid scientific advances lead to rapid development of engineering disciplines to assist in the understanding and application of this new knowledge. We are seeing this take place today in the development of the discipline of “bioengineering.”
My research is in the area of bioengineering. I have current interest/ projects in the broad areas of cell analysis/characterization/detection, cell separation, and cell culture, ranging from the study of human stem and cancer cells to industrially relevant cells. I also have interest in the emerging area of functional genomics, especially as it is related to cell culture, cell separation, and cancer cells.
VIDEO AT LEFT: confocal, microscopic images are shown of an actual circulating tumor cell isolated from the blood of a head and neck cancer patient. The nucleus is blue, and the green color is staining internal proteins consistent with a cancer cell.
Accordions
Jeffrey Chalmers
Ph.D., Cornell University, 1998
Professor Chalmers' research foci include iron metabolism, Intrinsic magnetization susceptibility of cells, cell separation, sickle cell detection/ treatment, anemia, immunomagnetic cell separation, cancer detection, and bioengineering.
Selected Achievements
- exploiting intrinsic magnetic susceptibility for cell characterization and separation
- characterization of the effect of hydrodynamic forces on cells
Major innovations in bioprocessing technologies for the 21st century, including:
- Further using an intrinsic properties of many cells, magnetic susceptibility, as both a signal to characterize the cells, and in a number of applications, separate the cells. A specific example, beyond focusing on red blood cells, is ferroptosis, an iron related cell death pathway that is gaining interest in cancer research.
- Progressively working towards the quantification of hydrodynamics forces in bioprocessing and its relationship to the hydrodynamic sensitivities frequently used in mammalian cell lines
- Designing a microfluidic apparatus that approximates the hydrodynamic conditions in bioprocess equipment through which shear sensitivity of different cell lines at different culture conditions can be quantified, enabling him to be among the first to quantitatively show that mammalians of these cell lines are very robust mechanically in the context of bioprocessing.
In the coming decades, cell separations will become increasingly important for the next wave of biologics, both for examining diseased cells as well as treating disease with cell therapies.
Chalmers has 13 patents.
Key Distinctions
International
Engineering Conference International:
- Cell Culture Engineering Award (bi-annual, 2014)
- Cell Culture Engineering Award lecture, 2016
National
American Association for the Advancement of Science:
- Fellow, 2005
American Institute of Chemical Engineers:
- Fellow, 2022
- 15C plenary lecture, 2005
American Institute for Medical and Biological Engineering:
- Fellow, 2001
National Science Foundation:
- Young Investigator Award, 1992-97
Regional
The Ohio State University
- Distinguished Scholar Award, 2023
Ohio Agricultural Research and Development Center and The Ohio State University
- Distinguished Multidisciplinary Team Research Award, 1996
The Ohio State University College of Engineering:
- Lumley Research Award, 2011, 2006, 2001, 1997, 1993
- Harrison Award, 2003
- Research Accomplishment Award, 1998
Additional Honors
Sigma Xi, Phi Beta Kappa, Tau Beta Pi:
- Member
Chronological List:
- Cell Culture Engineering Award lecture, May 2016
- Cell Culture Engineering Award, Engineering Conference International, 2014 (global, bi-annual award)
- College of Engineering Lumley Research Award: 2011, 2006, 2001, 1997, 1993
- Fellow, American Association for the Advancement of Science, 2005
- AIChE 15C plenary lecture, 2005
- Fellow, American Institute for Medical and Biological Engineering (AIMBE), 2001
- College of Engineering Annual Research Accomplishment Award, 1998
- Distinguished Multidisciplinary Team Research Award, 1996, Ohio Agricultural Research and Development Center and The Ohio State University
- NSF National Young Investigator Award, 1992-1997
- Member, Tau Beta Pi, Phi Beta Kappa, Sigma Xi
Testifying Expert Witness Matters
Applikon Biotechnology, Inc, vs United States, Case No 07-00364; January, 2011
Genentech Inc. vs Amgen, Case No. 17-1407-CFC; February, 2019
Regenlab USA LLC v. ESTAR TECHNOLOGIES, ECLIPSE AESTHETICS LLC, HEALEON Medical, INC., Case No. 1:16-cv-8771. July, 2019 (Deposed, and Markman hearing presentation)
Regenlab USA LLC v. ESTAR TECHNOLOGIES, ECLIPSE AESTHETICS LLC, HEALEON Medical, INC., Case No. 1:16-cv-8771. Sept., 2019
Non-Testifying
Harvest Technologies Corp. v. Cesca Therapeutics Inc. Case No. 12-1354; November 2014.
Amgen vs. AbbVie, January, 2016.
Samsung vs. Genentech, February, 2018.
Amgen vs. Genentech, January, 2019.
Research
When materials are exposed to an external magnetic field, the electrons' spins rearrange and generate magnetic dipole moments, which in turn affect the material's response to a magnetic field gradient. This leads to various magnetic properties, such as diamagnetism, paramagnetism, and ferromagnetism, determining the material's attraction or repulsion to the magnetic field.
For example, the bonds between hemoglobin and oxygen causes red blood cell to be repelled by magnetic field, inducing negative magnetic velocity. On the other hand, in deoxygenated red blood cells, the unpaired electrons can rearrange their spin following to the magnetic force, resulting positive magnetic velocity.
Since its high sensitivity, selectivity, and efficiency, the magnetic separation is highlighted for cell separation, detection and analysis.
Cell Separation
Based on the magnetic property of cell, we can perform cell separation and detection. The primary forces acting on the cells are magnetic force and gravity force, with additional reaction forces such as drag force and buoyancy force. In the presence of fluid flow within the channel, the inertia term associated with the flow is also taken into account.
Through the utilization of these physical properties, we are able to measure cell properties and enhance the efficiency of cell separation.
Our current focus areas are sickle blood cell, cancer cells, and glioblastoma.
Cell Tracking Velocimetry (CTV)
CTV is an innovative device that integrates a microscope camera with a microfluidic channel, creating a precisely controlled magnetic energy gradient. This setup allows for the precise tracking of cell and particle movements influenced by magnetic and gravitational fields.
Through a comparative analysis of settling velocity and magnetically induced velocity, we are able to investigate the properties of hemoglobin specifically associated with iron or magnetism.
Quadrupole Magnetic Sorter (QMS)
By strategically positioning Quadrupolar orientation magnets, we establish a specific magnetic field gradient within the device, facilitating continuous separation. The implementation of a continuous flow system further enables uninterrupted particle sorting.
By incorporating additional measurement devices into the outlets, real-time monitoring becomes feasible, paving the way for limitless future applications.
Magnetic nanoparticles (MNPs)
MNPs are cutting-edge materials that have recently gained significant attention in various fields, including chemical and biomedical engineering applications, due to their exceptional properties.
However, owing to the particles' nanometer-scale sizes, separating MNPs under the influence of a magnetic field is challenging, because Brownian motion impedes the separation of the desired particles from the surrounding medium.
We achieved successful separation of small SPIONs (5-30nm) by utilizing high magnetic fields and gradients. We also conducted in-situ Small Angle X-ray Scattering (SAXS) at Argonne National Laboratory to investigate the separation behavior for ferrofluid solution.
Predicitions
By utilizing COMSOL Multiphysics, a finite element analysis software designed for modeling and simulating physics-based phenomena, the magnetic fields and fluid flow velocities generated by different structures can be analyzed. These analyses serve as the basis for predicting particle movements.
The obtained simulation results play a crucial role in the development and scale up of highly efficient magnetic separators for cell separation.
Publications
Books
Zborowski, M. and Chalmers, J. J. Magnetic Cell Separation, Vol. 32 of Series: Laboratory Techniques in Biochemistry and Molecular Biology, Elsevier, Amsterdam, 2007.
Chapters
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Frank, G, Chalmers, J.J., Harrison, R.G., Todd, P.W., Rudge, S., Petrides, D.P., Pepper, C., Downey, B., Breit, J., Betenbaugh, M.J., Calzadilla, N. “Bioreactions and Bioprocessing.” Section 20, 9th Edition, Perry’s Chemical Engineers’ Handbook, In Press.
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Miller, B.L., Balasubramanian, P., Summers, T.A. Chalmers, J.J., Lustberg, M.B. “Spectral Deconvolution analysis of multicolor, fluorescently stained, negatively enriched, peripheral blood from cancer patients containing circulating tumor cells.” Submitted to Methods
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Frank, G, Chalmers, J.J., Harrison, R.G., Todd, P.W., Rudge, S., Petrides, D.P., Pepper, C., Downey, B., Breit, J., Betenbaugh, M.J., Calzadilla, N. “Bioreactions and Bioprocessing.” Section 20, 9th Edition, Perry’s Chemical Engineers’ Handbook, In Press (2016).
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Zborowski, M., Chalmers, J.J., “Magnetic Cell Manipulation and Sorting,” Microtechnology for Cell Manipulation and Sorting. W.Lee, P. Tseng, D. DiCarlo, eds., Springer (2017).
- Jeffrey J. Chalmers, Maryam B. Lustberg, Clayton Deighan, Kyoung-Joo Jenny Park, Yongqi Wu, Peter Amaya, Depletion of normal cells for CTC enrichment, Circulating Tumor Cells: Isolation and Analysis. John Wiley & Sons, 2015.
- Zborowski, M., Chalmers, J.J., Magnetophoresis: Fundamentals and Applications. Wiley Encyclopedia of Electrical and Electronics Engineering. John Wiley & Sons, 2015.
- Chalmers, J. J, Ma, N., Hydrodynamic Damage to Animal Cells, Cell Engineering, Animal Cell Culture, Vol 9:169-183. 2014.
- Lustberg, M., Jatana, K., Zborowski, M., Chalmers, J.J. Emerging Technologies for CTC detection Based on Depletion of Normal Cells. In: Minimal Residual Disease and Circulating Tumor Cells in Breast Cancer, Ed: K. Pantel, C. Sotiriou, M. Ignatiadis, Springer-Vertag GmbH, Berlin. 2012.
- Godoy-Silva, R., Berdogo, C., Chalmers, J.J. Aeration, Mixing, and Hydrodynamics in Bioreactors. In: Wiley Encyclopedia of Industrial Biotechnology, 2010.
- Xiong, Y. Shao, M., Zborowski, M., Chalmers, J.J. Magnetic cell separation to enrich for rare cells. In: Methods in Bioengineering (MIB), Vol. Editor K. Rege, Series editors: Yarmush, M and Langer, R.S. Artech House. 2009.
- Ma, Ningning, Mollet, M., Chalmers, J.J. Aeration, Mixing, and Hydrodynamics in Bioreactors. In: Cell Culture Technology for Pharmaceutical and Cellular Therapies. CRC Press, Taylor and Francis Group, Boca Raton, FL. 2006, Pp. 275-304.
- Lara, O. Chalmers, J.J. Cell enrichment and immunochemical staining. In: Immunochemical Protocols, Methods in Mol. Biol. Humana Press; Vol 295:301-10. 2005. PMID: 15596905.
- Zborowski, M., Chalmers, J.J. Magnetic Cell Sorting, In: Immunochemical Protocols, Methods in Mol. Biol. Humana Press; Vol 295:301-10. 2005.
- Zborowski, M., Williams, S, Chalmers, J.J., Magnetic FFF, Encyclopedia of Chromatography, Marcel Dekker, Inc. New York, 2001. Pp.503-507.
- Chalmers, J.J. Animal Cell Culture, Effects of Agitation and Aeration on Cell Adaptation, In: Encyclopedia of Cell Technology, R. Spier, J.B. Griffiths, A. H. Scragg, eds. 2000, Wiley, New York. Pp. 41-51.
- Zborowski, M., Moore, L.R., Sun, L., Chalmers, J.J., Continuous-flow Magnetic Cell Sorting Using Soluble Immunomagnetic Labels, In: Scientific and Clinical Applications of Magnetic Carriers, W. Schuett, U. Hafeli, J. Teller, M.Zborowski eds. (1997), Plenum Press.
- Chattopadyay, D., M. Garcia-Briones, R. Venkat, Chalmers, J.J., Hydrodynamic Properties in Bioreactors, In: Mammalian Cell Biotechnology in Protein Production, Hansjorg Hauser, Roland Wagner, (1997) ed., Walter de Gruyter, Publishers, Berlin.
- Chalmers, J.J., Shear Sensitivity of Insect Cells, In: Insect Cell Cultures: Fundamental and Applied Aspects, Just Vlak, et al. (eds). (1996) Kluwer Academic Publishers.
- Chalmers, J. J., The Effect of Hydrodynamic Forces on Insect Cells, In: Insect Cell Culture: Production of Improved Biopesticides and Proteins From Recombinant DNA, Michael Shuler (1995), ed. Hanser Publishers, New York.
- Shuler, M.L., Kim, B.G., Chalmers, J.J., Kim, E., Wilson, D.B. On Bioprocess Considerations and the Use of Genetically Modified Escherichia coli- systems for excretion of plasmic-encoded proteins, In: Bioprocess Engineering: 1st Generation, Trarum Ghose (1989), ed., Ellis Horwood Limited.
Visit Google Scholar for more information about publications from the Chalmers Laboratory.
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X. Wu, H. Choe, J. Strayer, J. Gómez-Pastora, M. Zborowski, B. Wyslouzil, and J. Chalmers
"Numerical modeling and in situ small angle X-ray scattering characterization of ultra-small SPION magnetophoresis in a high field and gradient separator"
Nanoscale, 2024.
https://doi.org/10.1039/D3NR05589B -
J. Strayer, H. Choe, X. Wu, M. Weigand,, M. Zborowski, and J. Chalmers
"Measuring magnetic force field distributions in microfluidic devices: Experimental and numerical approaches"
Electrophoresis, pp.1-9, 2023.
https://doi.org/10.1002/elps.202300093 -
L. R. Moore, N. A. Smith, S. J. Pike, M. Weigand, J. Gómez-Pastora, J. Strayer, A. F. Palmer, J. J. Chalmers, and M. Zborowski
"Red blood cell magnetophoresis as a function of oxygen partial pressure"
Journal of Magnetism and Magnetic Materials, vol. 564, pp. 169982, 2022.
https://doi.org/10.1016/j.jmmm.2022.169982 -
S. Lu, M. Allyn, M. Weigand, J. J. Chalmers, and A. F. Palmer
“Tangential flow filtration facilitated washing of human red blood cells: A proof‐of‐concept study”
Vox Sanguinis, vol. 117, no. 6, pp. 803-811, 2022.
https://doi.org/10.1111/vox.13259 - M. Weigand, J. Gómez-Pastora, J. Strayer, X. Wu, H. Choe, S. Lu, E. Plencner, K. Landes, A. Palmer, and M. Zborowski
“The Unique Magnetic Signature of Sickle Red Blood Cells: A Comparison Between the Red Blood Cells of Transfused and Non-Transfused Sickle Cell Disease Patients and Healthy Donors”
IEEE Transactions on Biomedical Engineering, vol. 69, no. 12, pp. 3582-3590, 2022.
https://doi.org/10.1109/TBME.2022.3172429 - M. Weigand, J. Gomez-Pastora, A. Palmer, M. Zborowski, P. Desai, and J. Chalmers
“Continuous-Flow Magnetic Fractionation of Red Blood Cells Based on Hemoglobin Content and Oxygen Saturation—Clinical Blood Supply Implications and Sickle Cell Anemia Treatment”
Processes, vol. 10, no. 5, pp. 927, 2022.
https://doi.org/10.3390/pr10050927 - J. Gómez-Pastora, M. Weigand, J. Kim, A. F. Palmer, M. Yazer, P. C. Desai, M. Zborowski, and J. J. Chalmers
“Potential of cell tracking velocimetry as an economical and portable hematology analyzer”
Scientific Reports, vol. 12, no. 1, pp. 1692, 2022.
https://doi.org/10.1038/s41598-022-05654-5 - X. Wu, J. Gómez-Pastora, M. Zborowski, and J. Chalmers
“SPIONs self-assembly and magnetic sedimentation in quadrupole magnets: Gaining insight into the separation mechanisms”
Separation and Purification Technology, vol. 280, pp. 119786, 2022.
https://doi.org/10.1016/j.seppur.2021.119786 -
C. González-Fernández, J. Gómez-Pastora, E. Bringas, M. Zborowski, J. J. Chalmers, and I. Ortiz
“Recovery of magnetic catalysts: advanced design for process intensification”
Industrial & Engineering Chemistry Research, vol. 60, no. 46, pp. 16780-16790, 2021.
https://doi.org/10.1021/acs.iecr.1c03474 -
J. J. Chalmers
“The challenges of hydrodynamic forces on cells used in cell manufacturing and therapy”
Current Opinion in Biomedical Engineering, vol. 20, pp. 100357, 2021.
https://doi.org/10.1016/j.cobme.2021.100357 - J. Gómez-Pastora, J. Kim, V. Multanen, M. Weigand, N. A. Walters, E. Reátegui, A. F. Palmer, M. H. Yazer, M. Zborowski, and J. J. Chalmers
“Intrinsically magnetic susceptibility in human blood and its potential impact on cell separation: Non-classical and intermediate monocytes have the strongest magnetic behavior in fresh human blood”
Experimental Hematology, vol. 99, pp. 21-31, 2021.
https://doi.org/10.1016/j.exphem.2021.05.003 - M. Weigand, J. Gómez-Pastora, J. Kim, M. T. Kurek, R. J. Hickey, D. C. Irwin, P. W. Buehler, M. Zborowski, A. F. Palmer, and J. J. Chalmers
“Magnetophoretic and spectral characterization of oxyhemoglobin and deoxyhemoglobin: Chemical versus enzymatic processes”
PLoS ONE, vol. 16, no. 9, pp. e0257061, 2021.
https://doi.org/10.1371/journal.pone.0257061 - C. González Fernández, J. Gómez Pastora, A. Basauri, M. Fallanza, E. Bringas, J. J. Chalmers, and I. Ortiz
“Continuous-flow separation of magnetic particles from biofluids: how does the microdevice geometry determine the separation performance?”
Sensors, vol. 20, no. 11, pp. 3030, 2020.
https://doi.org/10.3390/s20113030 - V. A. Roodan, J. Gómez-Pastora, I. H. Karampelas, C. González-Fernández, E. Bringas, I. Ortiz, J. J. Chalmers, E. P. Furlani, and M. T. Swihart
“Formation and manipulation of ferrofluid droplets with magnetic fields in a microdevice: a numerical parametric study”
Soft Matter, vol. 16, no. 41, pp. 9506-9518, 2020.
https://doi.org/10.1039/D0SM01426E - J. Gómez-Pastora, M. Weigand, J. Kim, X. Wu, J. Strayer, A. F. Palmer, M. Zborowski, M. Yazer, and J. J. Chalmers,
“Hyperferritinemia in critically ill COVID-19 patients–is ferritin the product of inflammation or a pathogenic mediator?”
Clinica Chimica Acta; International Journal of Clinical Chemistry, vol. 509, pp. 249, 2020.
https://doi.org/10.1016/j.cca.2020.06.033 - R. Wesolowski, D. G. Stover, M. B. Lustberg, A. Shoben, M. Zhao, E. Mrozek, R. M. Layman, E. Macrae, W. Duan, and J. Zhang
“Phase I Study of Veliparib on an Intermittent and Continuous Schedule in Combination with Carboplatin in Metastatic Breast Cancer: A Safety and [18F]‐Fluorothymidine Positron Emission Tomography Biomarker Study”
The Oncologist, vol. 25, no. 8, pp. e1158-e1169, 2020.
https://doi.org/10.1634/theoncologist.2020-0039 - K. D. Mahajan, G. Ruan, G. Vieira, T. Porter, J. J. Chalmers, R. Sooryakumar, and J. O. Winter
“Biomolecular detection, tracking, and manipulation using a magnetic nanoparticle-quantum dot platform”
Journal of Materials Chemistry B, vol. 8, no. 16, pp. 3534-3541, 2020.
https://doi.org/10.1039/C9TB02481F - J. Kim, J. Gómez-Pastora, C. J. Gilbert, M. Weigand, N. A. Walters, E. Reátegui, A. F. Palmer, M. Yazer, M. Zborowski, and J. J. Chalmers
“Quantification of the mean and distribution of hemoglobin content in normal human blood using cell tracking velocimetry”
Analytical Chemistry, vol. 92, no. 2, pp. 1956-1962, 2019.
https://doi.org/10.1021/acs.analchem.9b04302 - J. Kim, J. Gómez‐Pastora, M. Weigand, M. Potgieter, N. A. Walters, E. Reátegui, A. F. Palmer, M. Yazer, M. Zborowski, and J. J. Chalmers
“A subpopulation of monocytes in normal human blood has significant magnetic susceptibility: Quantification and potential implications”
Cytometry Part A, vol. 95, no. 5, pp. 478-487, 2019.
https://doi.org/10.1002/cyto.a.23755 - K. J. J. Park, J. Kim, T. Testoff, J. Adams, M. Poklar, M. Zborowski, M. Venere, and J. J. Chalmers
“Quantitative characterization of the regulation of iron metabolism in glioblastoma stem‐like cells using magnetophoresis”
Biotechnology and Bioengineering, vol. 116, no. 7, pp. 1644-1655, 2019.
https://doi.org/10.1002/bit.26973 - J. Kim, M. Weigand, A. F. Palmer, M. Zborowski, M. H. Yazer, and J. J. Chalmers
“Single cell analysis of aged RBCs: quantitative analysis of the aged cells and byproducts”
Analyst, vol. 144, no. 3, pp. 935-942, 2019.
https://doi.org/10.1039/C8AN01904E - W. Xue, L. R. Moore, N. Nakano, J. J. Chalmers, and M. Zborowski
“Single cell magnetometry by magnetophoresis vs. bulk cell suspension magnetometry by SQUID-MPMS–A comparison”
Journal of Magnetism and Magnetic Materials, vol. 474, pp. 152-160, 2019.
https://doi.org/10.1016/j.jmmm.2018.10.108 - M. B. Lustberg, D. G. Stover, and J. J. Chalmers
“Implementing liquid biopsies in clinical trials: State of affairs, opportunities and challenges”
Cancer Journal (Sudbury, Mass.), vol. 24, no. 2, pp. 61, 2018.
https://doi.org/10.1097/PPO.0000000000000309 - H. M. Aljohani, M. Aittaleb, J. M. Furgason, P. Amaya, A. Deeb, J. J. Chalmers, and E. M. Bahassi
“Genetic mutations associated with lung cancer metastasis to the brain”
Mutagenesis, vol. 33, no. 2, pp. 137-145, 2018.
https://doi.org/10.1093/mutage/gey003 - L. R. Moore, D. Mizutani, T. Tanaka, A. Buck, M. Yazer, M. Zborowski, and J. J. Chalmers
“Continuous, intrinsic magnetic depletion of erythrocytes from whole blood with a quadrupole magnet and annular flow channel; pilot scale study”
Biotechnology and Bioengineering, vol. 115, no. 6, pp. 1521-1530, 2018.
https://doi.org/10.1002/bit.26581 - J. Sun, L. Moore, W. Xue, J. Kim, M. Zborowski, and J. J. Chalmers
“Correlation of simulation/finite element analysis to the separation of intrinsically magnetic spores and red blood cells using a microfluidic magnetic deposition system”
Biotechnology and Bioengineering, vol. 115, no. 5, pp. 1288-1300, 2018.
https://doi.org/10.1002/bit.26550 - K. D. Mahajan, Y. Cui, C. J. Dorcéna, N. F. Bouxsien, G. D. Bachand, J. J. Chalmers, and J. O. Winter
“Magnetic Quantum Dots Steer and Detach Microtubules From Kinesin‐Coated Surfaces”
Biotechnology Journal, vol. 13, no. 1, pp. 1700402, 2018.
https://doi.org/10.1002/biot.201700402 - K. D. Mahajan, G. M. Nabar, W. Xue, M. Anghelina, N. I. Moldovan, J. J. Chalmers, and J. O. Winter
“Mechanotransduction effects on endothelial cell proliferation via CD31 and VEGFR2: implications for immunomagnetic separation”
Biotechnology Journal, vol. 12, no. 9, pp. 1600750, 2017. - D. S. Sutaria, J. Jiang, O. A. Elgamal, S. M. Pomeroy, M. Badawi, X. Zhu, R. Pavlovicz, A. C. P. Azevedo-Pouly, J. Chalmers, and C. Li
“Low active loading of cargo into engineered extracellular vesicles results in inefficient miRNA mimic delivery”
Journal of Extracellular Vesicles, vol. 6, no. 1, pp. 1333882, 2017. - J. J. Chalmers, X. Jin, A. F. Palmer, M. H. Yazer, L. Moore, P. Amaya, K. Park, X. Pan, and M. Zborowski
“Femtogram resolution of iron content on a per cell basis: ex vivo storage of human red blood cells leads to loss of hemoglobin”
Analytical Chemistry, vol. 89, no. 6, pp. 3702-3709, 2017. - L. R. Moore, P. S. Williams, J. J. Chalmers, and M. Zborowski
“Tessellated permanent magnet circuits for flow-through, open gradient separations of weakly magnetic materials”
Journal of Magnetism and Magnetic Materials, vol. 427, pp. 325-330, 2017. - Y. Wu, K.-J. J. Park, C. Deighan, P. Amaya, B. Miller, Q. Pan, M. Zborowski, M. Lustberg, and J. Chalmers
“Multiparameter evaluation of the heterogeneity of circulating tumor cells using integrated RNA in situ hybridization and immunocytochemical analysis”
Frontiers in Oncology, vol. 6, pp. 234, 2016. - K. D. Mahajan, G. Ruan, C. J. Dorcéna, G. Vieira, G. Nabar, N. F. Bouxsein, J. J. Chalmers, G. D. Bachand, R. Sooryakumar, and J. O. Winter
“Steering microtubule shuttle transport with dynamically controlled magnetic fields”
Nanoscale, vol. 8, no. 16, pp. 8641-8649, 2016. - K. P. McMullen, J. J. Chalmers, J. C. Lang, P. Kumar, and K. R. Jatana
“Circulating tumor cells in head and neck cancer: A review”
World Journal of Otorhinolaryngology-Head and Neck Surgery, vol. 2, no. 02, pp. 109-116, 2016. - K. R. Jatana, P. Balasubramanian, K. P. McMullen, J. C. Lang, T. N. Teknos, and J. J. Chalmers
“Effect of surgical intervention on circulating tumor cells in patients with squamous cell carcinoma of the head and neck using a negative enrichment technology”
Head & Neck, vol. 38, no. 12, pp. 1799-1803, 2016. - J. J. Gallardo-Rodríguez, L. López-Rosales, A. Sánchez-Mirón, F. García-Camacho, E. Molina-Grima, and J. J. Chalmers
“New insights into shear-sensitivity in dinoflagellate microalgae”
Bioresource Technology, vol. 200, pp. 699-705, 2016. - J. J. Chalmers
“Mixing, aeration and cell damage, 30+ years later: what we learned, how it affected the cell culture industry and what we would like to know more about”
Current Opinion in Chemical Engineering, vol. 10, pp. 94-102, 2015. - J. P. Oliveira-Costa, A. F. de Carvalho, G. G. da Silveira, P. Amaya, Y. Wu, K.-J. J. Park, M. P. Gigliola, M. Lustberg, M. E. C. Buim, and E. N. Ferreira
“Gene expression patterns through oral squamous cell carcinoma development: PD-L1 expression in primary tumor and circulating tumor cells”
Oncotarget, vol. 6, no. 25, pp. 20902, 2015. - M. Barrow, A. Taylor, D. J. Nieves, L. K. Bogart, P. Mandal, C. M. Collins, L. R. Moore, J. J. Chalmers, R. Lévy, and S. R. Williams
“Tailoring the surface charge of dextran-based polymer coated SPIONs for modulated stem cell uptake and MRI contrast”
Biomaterials science, vol. 3, no. 4, pp. 608-616, 2015. - A. Buck, L. R. Moore, C. D. Lane, A. Kumar, C. Stroff, N. White, W. Xue, J. J. Chalmers, and M. Zborowski
“Magnetic separation of algae genetically modified for increased intracellular iron uptake”
Journal of Magnetism and Magnetic Materials, vol. 380, pp. 201-204, 2015. - H. Mathsyaraja, K. Thies, D. A. Taffany, C. Deighan, T. Liu, L. Yu, S. A. Fernandez, C. Shapiro, J. Otero, and C. Timmers
“CSF1-ETS2-induced microRNA in myeloid cells promote metastatic tumor growth”
Oncogene, vol. 34, no. 28, pp. 3651-3661, 2015. - L. R. Moore, P. S. Williams, F. Nehl, K. Abe, J. J. Chalmers, and M. Zborowski
“Feasibility study of red blood cell debulking by magnetic field-flow fractionation with step-programmed flow”
Analytical and Bioanalytical Chemistry, vol. 406, pp. 1661-1670, 2014. - M. B. Lustberg, P. Balasubramanian, B. Miller, A. Garcia-Villa, C. Deighan, Y. Wu, S. Carothers, M. Berger, B. Ramaswamy, and E. R. Macrae
“Heterogeneous atypical cell populations are present in blood of metastatic breast cancer patients”
Breast Cancer Research, vol. 16, no. 2, pp. 1-15, 2014. - Y. Wu, C. J. Deighan, B. L. Miller, P. Balasubramanian, M. B. Lustberg, M. Zborowski, and J. J. Chalmers
“Isolation and analysis of rare cells in the blood of cancer patients using a negative depletion methodology,”
Methods, vol. 64, no. 2, pp. 169-182, 2013. - A. Chen, T. Byvank, W.-J. Chang, A. Bharde, G. Vieira, B. L. Miller, J. J. Chalmers, R. Bashir, and R. Sooryakumar
“On-chip magnetic separation and encapsulation of cells in droplets”
Lab on a Chip, vol. 13, no. 6, pp. 1172-1181, 2013. - L. R. Moore, F. Nehl, J. Dorn, J. J. Chalmers, and M. Zborowski
“Open gradient magnetic red blood cell sorter evaluation on model cell mixtures”
IEEE Transactions on Magnetics, vol. 49, no. 1, pp. 309-315, 2012. - K. D. Mahajan, G. B. Vieira, G. Ruan, B. L. Miller, M. B. Lustberg, J. J. Chalmers, R. Sooryakumar, and J. O. Winter
“A MagDot-nanoconveyor assay detects and isolates molecular biomarkers”
Chemical Engineering Progress, vol. 108, pp. 41, 2012. - J. J. Chalmers
"Magnetic, Batch Separation"
Methods in Cell Biology, pp. 193-201: Elsevier, 2012. - B. L. Miller and J. J. Chalmers
"Cell Enrichment from human blood through red cell lysis"
Methods in Cell Biology, pp. 183-192: Elsevier, 2012. - J. Xu, K. Mahajan, W. Xue, J. O. Winter, M. Zborowski, and J. J. Chalmers
“Simultaneous, single particle, magnetization and size measurements of micron sized, magnetic particles”
Journal of Magnetism and Magnetic Materials, vol. 324, no. 24, pp. 4189-4199, 2012. - A. Garcia-Villa, P. Balasubramanian, B. L. Miller, M. B. Lustberg, B. Ramaswamy, and J. J. Chalmers
“Assessment of γ-H2AX levels in circulating tumor cells from patients receiving chemotherapy”
Frontiers in Oncology, vol. 2, pp. 128, 2012. -
M. B. Lustberg, B. L. Miller, Y. Wu, W. Xue, C. Deighan, S. Carothers, M. J. Berger, C. L. Shapiro, and J. J. Chalmers
“Circulating CD68 positive (+) leukocytes in blood samples from patients (pts) with breast cancer (BC)”
Journal of Clinical Oncology, vol. 30, no. 27_suppl, pp. 172-172, 2012. -
X. Jin, S. Abbot, X. Zhang, L. Kang, V. Voskinarian-Berse, R. Zhao, M. V. Kameneva, L. R. Moore, J. J. Chalmers, and M. Zborowski
“Erythrocyte enrichment in hematopoietic progenitor cell cultures based on magnetic susceptibility of the hemoglobin”
PLoS ONE, vol. 7, no. 8, pp. e39491, 2012. -
X. Jin, J. J. Chalmers, and M. Zborowski,
Iron transport in cancer cell culture suspensions measured by cell magnetophoresis”
Analytical Chemistry, vol. 84, no. 10, pp. 4520-4526, 2012. -
M. Lustberg, K. R. Jatana, M. Zborowski, and J. J. Chalmers
“Emerging technologies for CTC detection based on depletion of normal cells”
Minimal Residual Disease and Circulating Tumor Cells in Breast Cancer, pp. 97-110, 2012. -
X. Jin, M. H. Yazer, J. J. Chalmers, and M. Zborowski
“Quantification of changes in oxygen release from red blood cells as a function of age based on magnetic susceptibility measurements”
Analyst, vol. 136, no. 14, pp. 2996-3003, 2011. -
Y. Wu, N. Ma, B. E. Wyslouzil, J. J. Chalmers, E. McCormick, and S. A. Casnocha
“Enhanced productivity of NS0 cells in fed‐batch culture with cholesterol nanoparticle supplementation”
Biotechnology Progress, vol. 27, no. 3, pp. 796-802, 2011. -
J. Sun, M. Zborowski, and J. J. Chalmers
“Quantification of both the presence, and oxidation state, of Mn in Bacillus atrophaeus spores and its imparting of magnetic susceptibility to the spores”
Biotechnology and Bioengineering, vol. 108, no. 5, pp. 1119-1129, 2011. -
K. R. Jatana, J. C. Lang, and J. J. Chalmers
“Identification of circulating tumor cells: a prognostic marker in squamous cell carcinoma of the head and neck?”
Future Oncology, vol. 7, no. 4, pp. 481-484, 2011. -
W. Hu, R. Gladue, J. Hansen, C. Wojnar, and J. J. Chalmers
“Growth inhibition of dinoflagellate algae in shake flasks: Not due to shear this time!”
Biotechnology Progress, vol. 26, no. 1, pp. 79-87, 2010. -
T. Henighan, A. Chen, G. Vieira, A. J. Hauser, F. Y. Yang, J. J. Chalmers, and R. Sooryakumar
“Manipulation of magnetically labeled and unlabeled cells with mobile magnetic traps”
Biophysical Journal, vol. 98, no. 3, pp. 412-417, 2010. -
T. Schneider, S. Karl, L. R. Moore, J. J. Chalmers, P. S. Williams, and M. Zborowski
“Sequential CD34 cell fractionation by magnetophoresis in a magnetic dipole flow sorter”
Analyst, vol. 135, no. 1, pp. 62-70, 2010. -
J. J. Chalmers, Y. Xiong, X. Jin, M. Shao, X. Tong, S. Farag, and M. Zborowski
“Quantification of non‐specific binding of magnetic micro‐and nanoparticles using cell tracking velocimetry: Implication for magnetic cell separation and detection”
Biotechnology and Bioengineering, vol. 105, no. 6, pp. 1078-1093, 2010. -
Y. Wu, J. J. Chalmers, and B. E. Wyslouzil
“The use of electrohydrodynamic spraying to disperse hydrophobic compounds in aqueous media”
Aerosol Science and Technology, vol. 43, no. 9, pp. 902-910, 2009. -
P. Balasubramanian, L. Yang, J. C. Lang, K. R. Jatana, D. Schuller, A. Agrawal, M. Zborowski, and J. J. Chalmers
“Confocal images of circulating tumor cells obtained using a methodology and technology that removes normal cells”
Molecular Pharmaceutics, vol. 6, no. 5, pp. 1402-1408, 2009. -
R. Godoy‐Silva, J. J. Chalmers, S. A. Casnocha, L. A. Bass, and N. Ma
“Physiological responses of CHO cells to repetitive hydrodynamic stress”
Biotechnology and Bioengineering, vol. 103, no. 6, pp. 1103-1117, 2009. -
R. M. Shenkman, J. J. Chalmers, B. J. Hering, N. Kirchhof, and K. K. Papas
“Quadrupole magnetic sorting of porcine islets of Langerhans”
Tissue Engineering Part C: Methods, vol. 15, no. 2, pp. 147-156, 2009. -
R. M. Shenkman, R. Godoy‐Silva, K. K. Papas, and J. J. Chalmers
“Effects of energy dissipation rate on islets of Langerhans: implications for isolation and transplantation”
Biotechnology and Bioengineering, vol. 103, no. 2, pp. 413-423, 2009. -
R. Godoy‐Silva, M. Mollet, and J. J. Chalmers
“Evaluation of the effect of chronic hydrodynamical stresses on cultures of suspensed CHO‐6E6 cells”
Biotechnology and Bioengineering, vol. 102, no. 4, pp. 1119-1130, 2009. -
Y. Jing, N. Mal, P. S. Williams, M. Mayorga, M. S. Penn, J. J. Chalmers, and M. Zborowski
“Quantitative intracellular magnetic nanoparticle uptake measured by live cell magnetophoresis”
The FASEB Journal, vol. 22, no. 12, pp. 4239-4247, 2008. -
X. Jin, Y. Zhao, A. Richardson, L. Moore, P. S. Williams, M. Zborowski, and J. J. Chalmers
“Differences in magnetically induced motion of diamagnetic, paramagnetic, and superparamagnetic microparticles detected by cell tracking velocimetry”
Analyst, vol. 133, no. 12, pp. 1767-1775, 2008. -
D. F. Farson, H. W. Choi, B. Zimmerman, J. K. Steach, J. J. Chalmers, S. V. Olesik, and L. J. Lee
“Femtosecond laser micromachining of dielectric materials for biomedical applications”
Journal of Micromechanics and Microengineering, vol. 18, no. 3, pp. 035020, 2008. -
M. Mollet, R. Godoy‐Silva, C. Berdugo, and J. J. Chalmers
“Computer simulations of the energy dissipation rate in a fluorescence‐activated cell sorter: Implications to cells”
Biotechnology and Bioengineering, vol. 100, no. 2, pp. 260-272, 2008. -
W. Hu, J. J. Rathman, and J. J. Chalmers
“An investigation of small‐molecule surfactants to potentially replace pluronic F‐68 for reducing bubble‐associated cell damage”
Biotechnology and Bioengineering, vol. 101, no. 1, pp. 119-127, 2008. -
W. Hu, R. Gladue, J. Hansen, C. Wojnar, and J. J. Chalmers
“The sensitivity of the dinoflagellate Crypthecodinium cohnii to transient hydrodynamic forces and cell‐bubble interactions”
Biotechnology Progress, vol. 23, no. 6, pp. 1355-1362, 2007. -
X. Tong, Y. Xiong, M. Zborowski, S. S. Farag, and J. J. Chalmers
“A novel high throughput immunomagnetic cell sorting system for potential clinical scale depletion of T cells for allogeneic stem cell transplantation”
Experimental Hematology, vol. 35, no. 10, pp. 1613-1622, 2007. -
X. Tong, L. Yang, J. C. Lang, M. Zborowski, and J. J. Chalmers
“Application of immunomagnetic cell enrichment in combination with RT‐PCR for the detection of rare circulating head and neck tumor cells in human peripheral blood”
Cytometry Part B: Clinical Cytometry, vol. 72, no. 5, pp. 310-323, 2007. -
M. Mollet, R. Godoy‐Silva, C. Berdugo, and J. J. Chalmers
“Acute hydrodynamic forces and apoptosis: a complex question”
Biotechnology and Bioengineering, vol. 98, no. 4, pp. 772-788, 2007. -
B. C. Mehta, D. W. Holman, D. M. Grzybowski, and J. J. Chalmers
“Characterization of arachnoidal cells cultured on three-dimensional nonwoven PET matrix”
Tissue Engineering, vol. 13, no. 6, pp. 1269-1279, 2007. -
Y. Jing, L. R. Moore, T. Schneider, P. S. Williams, J. J. Chalmers, S. S. Farag, B. Bolwell, and M. Zborowski
“Negative selection of hematopoietic progenitor cells by continuous magnetophoresis”
Experimental Hematology, vol. 35, no. 4, pp. 662-672, 2007. -
K. Melnik, J. Sun, A. Fleischman, S. Roy, M. Zborowski, and J. J. Chalmers
“Quantification of magnetic susceptibility in several strains of Bacillus spores: Implications for separation and detection”
Biotechnology and Bioengineering, vol. 98, no. 1, pp. 186-192, 2007. -
T. Schneider, L. R. Moore, Y. Jing, S. Haam, P. S. Williams, A. J. Fleischman, S. Roy, J. J. Chalmers, and M. Zborowski
“Continuous flow magnetic cell fractionation based on antigen expression level”
Journal of Biochemical and Biophysical Methods, vol. 68, no. 1, pp. 1-21, 2006. -
O. Lara, X. Tong, M. Zborowski, S. S. Farag, and J. J. Chalmers
“Comparison of two immunomagnetic separation technologies to deplete T cells from human blood samples”
Biotechnology and Bioengineering, vol. 94, no. 1, pp. 66-80, 2006. -
H. Zhang, P. S. Williams, M. Zborowski, and J. J. Chalmers
“Binding affinities/avidities of antibody–antigen interactions: Quantification and scale‐up implications”
Biotechnology and Bioengineering, vol. 95, no. 5, pp. 812-829, 2006. -
D. R. Leigh, S. Steinert, L. R. Moore, J. J. Chalmers, and M. Zborowski
“Cell tracking velocimetry as a tool for defining saturation binding of magnetically conjugated antibodies”
Cytometry Part A, vol. 66, no. 2, pp. 103-108, 2005. -
F. Carpino, L. R. Moore, M. Zborowski, J. J. Chalmers, and P. S. Williams
“Analysis of magnetic nanoparticles using quadrupole magnetic field-flow fractionation”
Journal of Magnetism and Magnetic Materials, vol. 293, no. 1, pp. 546-552, 2005. -
H. Zhang, L. R. Moore, M. Zborowski, P. S. Williams, S. Margel, and J. J. Chalmers
“Establishment and implications of a characterization method for magnetic nanoparticle using cell tracking velocimetry and magnetic susceptibility modified solutions”
Analyst, vol. 130, no. 4, pp. 514-527, 2005. -
L. R. Moore, S. Milliron, P. S. Williams, J. J. Chalmers, S. Margel, and M. Zborowski
“Control of magnetophoretic mobility by susceptibility-modified solutions as evaluated by cell tracking velocimetry and continuous magnetic sorting”
Analytical Chemistry, vol. 76, no. 14, pp. 3899-3907, 2004. -
N. Ma, J. J. Chalmers, J. G. Auniņš, W. Zhou, and L. Xie
“Quantitative studies of cell‐bubble interactions and cell damage at different Pluronic F‐68 and cell concentrations”
Biotechnology Progress, vol. 20, no. 4, pp. 1183-1191, 2004. -
J. P. Fife, R. C. Derksen, H. E. Ozkan, P. S. Grewal, J. J. Chalmers, and C. R. Krause
“Evaluation of a contraction flow field on hydrodynamic damage to entomopathogenic nematodes—a biological pest control agent”
Biotechnology and Bioengineering, vol. 86, no. 1, pp. 96-107, 2004. -
P. S. Williams, K. Decker, M. Nakamura, J. J. Chalmers, L. R. Moore, and M. Zborowski
“Splitter imperfections in annular split-flow thin separation channels: experimental study of nonspecific crossover”
Analytical Chemistry, vol. 75, no. 23, pp. 6687-6695, 2003. -
P. S. Williams, L. R. Moore, J. J. Chalmers, and M. Zborowski
“Splitter imperfections in annular split-flow thin separation channels: effect on nonspecific crossover”
Analytical Chemistry, vol. 75, no. 6, pp. 1365-1373, 2003. -
K. E. McCloskey, L. R. Moore, M. Hoyos, A. Rodriguez, J. J. Chalmers, and M. Zborowski, “Magnetophoretic cell sorting is a function of antibody binding capacity”
Biotechnology Progress, vol. 19, no. 3, pp. 899-907, 2003. -
E. J. Chosy, M. Nakamura, K. Melnik, K. Comella, L. C. Lasky, M. Zborowski, and J. J. Chalmers
“Characterization of antibody binding to three cancer‐related antigens using flow cytometry and cell tracking velocimetry”
Biotechnology and Bioengineering, vol. 82, no. 3, pp. 340-351, 2003. -
M. Zborowski, L. R. Moore, P. S. Williams, and J. J. Chalmers
“Separations based on magnetophoretic mobility”
Separation Science and Technology, vol. 37, no. 16, pp. 3611-3633, 2002. -
M. Hoyos, K. E. McCloskey, L. R. Moore, M. Nakamura, B. J. Bolwell, J. J. Chalmers, and M. Zborowski
“Pulse-injection studies of blood progenitor cells in a quadrupole magnet flow sorter”
Separation Science and Technology, vol. 37, no. 4, pp. 745-767, 2002. -
K. Comella, M. Nakamura, K. Melnik, J. Chosy, M. Zborowski, M. A. Cooper, T. A. Fehniger, M. A. Caligiuri, and J. J. Chalmers
“Effects of antibody concentration on the separation of human natural killer cells in a commercial immunomagnetic separation system”
Cytometry, vol. 45, no. 4, pp. 285-293, 2001. -
K. Melnik, M. Nakamura, K. Comella, L. C. Lasky, M. Zborowski, and J. J. Chalmers
“Evaluation of eluents from separations of CD34+ cells from human cord blood using a commerical, immunomagnetic cell separation system”
Biotechnology Progress, vol. 17, no. 5, pp. 907-916, 2001. -
K. E. McCloskey, K. Comella, J. J. Chalmers, S. Margel, and M. Zborowski
“Mobility measurements of immunomagnetically labeled cells allow quantitation of secondary antibody binding amplification”
Biotechnology and Bioengineering, vol. 75, no. 6, pp. 642-655, 2001. -
M. Nakamura, K. Decker, J. Chosy, K. Comella, K. Melnik, L. Moore, L. C. Lasky, M. Zborowski, and J. J. Chalmers
“Separation of a breast cancer cell line from human blood using a quadrupole magnetic flow sorter”
Biotechnology Progress, vol. 17, no. 6, pp. 1145-1155, 2001. -
K. E. McCloskey, M. Zborowski, and J. J. Chalmers
“Measurement of CD2 expression levels of IFN‐α–treated fibrosarcomas using cell tracking velocimetry”
Cytometry, vol. 44, no. 2, pp. 137-147, 2001. -
L. R. Moore, A. R. Rodriguez, P. S. Williams, K. McCloskey, B. J. Bolwell, M. Nakamura, J. J. Chalmers, and M. Zborowski
“Progenitor cell isolation with a high-capacity quadrupole magnetic flow sorter”
Journal of Magnetism and Magnetic Materials, vol. 225, no. 1-2, pp. 277-284, 2001. -
M. Nakamura, M. Zborowski, L. C. Lasky, S. Margel, and J. J. Chalmers
“Theoretical and experimental analysis of the accuracy and reproducibility of cell tracking velocimetry”
Experiments in Fluids, vol. 30, no. 4, pp. 371-380, 2001. -
M. Hoyos, L. R. Moore, K. E. McCloskey, S. Margel, M. Zuberi, J. J. Chalmers, and M. Zborowski
“Study of magnetic particles pulse-injected into an annular SPLITT-like channel inside a quadrupole magnetic field”
Journal of Chromatography A, vol. 903, no. 1-2, pp. 99-116, 2000. -
L. R. Moore, M. Zborowski, M. Nakamura, K. McCloskey, S. Gura, M. Zuberi, S. Margel, and J. J. Chalmers
“The use of magnetite-doped polymeric microspheres in calibrating cell tracking velocimetry”
Journal of Biochemical and Biophysical Methods, vol. 44, no. 1-2, pp. 115-130, 2000. - K. E. McCloskey, J. J. Chalmers, and M. Zborowski
“Magnetophoretic mobilities correlate to antibody binding capacities”
Cytometry, vol. 40, no. 4, pp. 307-315, 2000. - P. Bauer, L. Hancock, J. Rathman, and J. J. Chalmers
“Cell‐microcarrier adhesion to gas‐liquid interfaces and foam”
Biotechnology Progress, vol. 16, no. 1, pp. 125-132, 2000. - P. S. Williams, M. Zborowski, and J. J. Chalmers
“Flow rate optimization for the quadrupole magnetic cell sorter”
Analytical Chemistry, vol. 71, no. 17, pp. 3799-3807, 1999. - J. J. Chalmers, S. Haam, Y. Zhao, K. McCloskey, L. Moore, M. Zborowski, and P. S. Williams
“Quantification of cellular properties from external fields and resulting induced velocity: cellular hydrodynamic diameter”
Biotechnology and Bioengineering, vol. 64, no. 5, pp. 509-518, 1999. - J. J. Chalmers, S. Haam, Y. Zhao, K. McCloskey, L. Moore, M. Zborowski, and P. S. Williams
“Quantification of cellular properties from external fields and resulting induced velocity: magnetic susceptibility”
Biotechnology and Bioengineering, vol. 64, no. 5, pp. 519-526, 1999. - M. Zborowski, L. Sun, L. R. Moore, and J. J. Chalmers
“Rapid cell isolation by magnetic flow sorting for applications in tissue engineering”
ASAIO Journal (American Society for Artificial Internal Organs: 1992), vol. 45, no. 3, pp. 127-130, 1999. - L. R. Moore, M. Zborowski, L. Sun, and J. J. Chalmers
“Lymphocyte fractionation using immunomagnetic colloid and a dipole magnet flow cell sorter”
Journal of Biochemical and Biophysical Methods, vol. 37, no. 1-2, pp. 11-33, 1998. - J. J. Chalmers
“Gas Bubbles and Their Influence on Microorganisms”
Applied Mechanics Reviews, vol. 51, no. 1, pp. 113-120, 1998. - G. W. Wheeler, D. L. Tomasko, and J. J. Chalmers
“Dynamics of a linked supercritical extraction-biodegradation process for organic wastes”
Chemical Engineering Science, vol. 53, no. 2, pp. 189-201, 1998. - J. J. Chalmers, M. Zborowski, L. Moore, S. Mandal, B. Fang, and L. Sun
“Theoretical analysis of cell separation based on cell surface marker density”
Biotechnology and Bioengineering, vol. 59, no. 1, pp. 10-20, 1998. - L. Sun, M. Zborowski, L. R. Moore, and J. J. Chalmers
“Continuous, flow‐through immunomagnetic cell sorting in a quadrupole field”
Cytometry, vol. 33, no. 4, pp. 469-475, 1998. - J. J. Chalmers, C. Mauras, and R. Vir
“Confocal Microscopic Images of a Compost Particle”
Biotechnology Progress, vol. 13, no. 6, pp. 727-732, 1997. - M. Zborowski, P. S. Williams, L. Sun, L. R. Moore, and J. J. Chalmers
“Cylindrical SPLITT and quadrupole magnetic field in application to continuous-flow magnetic cell sorting”
Journal of Liquid Chromatography & Related Technologies, vol. 20, no. 16-17, pp. 2887-2905, 1997. - J. J. Chalmers
“Shear sensitivity of insect cells”
Cytotechnology, vol. 20, pp. 163-171, 1996. - D. Y. Tseng, R. Vir, S. J. Traina, and J. J. Chalmers
“A Fourier‐transform infrared spectroscopic analysis of organic matter degradation in a bench‐scale solid substrate fermentation (composting) system”
Biotechnology and Bioengineering, vol. 52, no. 6, pp. 661-671, 1996. - D. Y. Tseng, J. J. Chalmers, and O. H. Tuovinen
“ATP measurement in compost”
Compost Science & Utilization, vol. 4, no. 3, pp. 6-17, 1996. - M. Zborowski, C. B. Fuh, R. Green, N. J. Baldwin, S. Reddy, T. Douglas, S. Mann, and J. J. Chalmers
“Immunomagnetic isolation of magnetoferritin‐labeled cells in a modified ferrograph”
Cytometry, vol. 24, no. 3, pp. 251-259, 1996. - R. V. Venkat, L. R. Stock, and J. J. Chalmers
“Study of hydrodynamics in microcarrier culture spinner vessels: a particle tracking velocimetry approach”
Biotechnology and Bioengineering, vol. 49, no. 4, pp. 456-466, 1996. - S. Reddy, L. R. Moore, L. Sun, M. Zborowski, and J. J. Chalmers
“Determination of the magnetic susceptibility of labeled particles by video imaging”
Chemical Engineering Science, vol. 51, no. 6, pp. 947-956, 1996. - R. V. Venkat, and J. J. Chalmers
“Characterization of agitation environments in 250 ml spinner vessel, 3 L, and 20 L reactor vessels used for animal cell microcarrier culture”
Cytotechnology, vol. 22, pp. 95-102, 1996. - D. Y. Tseng, J. J. Chalmers, O. H. Tuovinen, and H. A. J. Hoitink
“Characterization of a bench-scale system for studying the biodegradation of organic solid wastes”
Biotechnology Progress, vol. 11, no. 4, pp. 443-451, 1995. - D. Chattopadhyay, J. F. Rathman, and J. J. Chalmers
“Thermodynamic approach to explain cell adhesion to air–medium interfaces”
Biotechnology and Bioengineering, vol. 48, no. 6, pp. 649-658, 1995. - D. Chattopadhyay, J. F. Rathman, and J. J. Chalmers
“The protective effect of specific medium additives with respect to bubble rupture”
Biotechnology and Bioengineering, vol. 45, no. 6, pp. 473-480, 1995. - M. A. Garcia-Briones, R. S. Brodkey, and J. J. Chalmers
“Computer simulations of the rupture of a gas bubble at a gas—liquid interface and its implications in animal cell damage”
Chemical Engineering Science, vol. 49, no. 14, pp. 2301-2320, 1994. - J. J. Chalmers
“Cells and bubbles in sparged bioreactors”
Cell Culture Engineering IV: Improvements of Human Health, pp. 311-320, 1995. - M. A. Garcia‐Briones, and J. J. Chalmers
“Flow parameters associated with hydrodynamic cell injury”
Biotechnology and Bioengineering, vol. 44, no. 9, pp. 1089-1098, 1994. - K. Trinh, M. Garcia‐Briones, J. J. Chalmers, and F. Hink
“Quantification of damage to suspended insect cells as a result of bubble rupture”
Biotechnology and Bioengineering, vol. 43, no. 1, pp. 37-45, 1994. - M. Garcia‐Briones, and J. J. Chalmers
“Cell‐bubble interactions: mechanisms of suspended cell damage”
Annals of the New York Academy of Sciences, vol. 665, no. 1, pp. 219-229, 1992. - G. L. Kleman, J. J. Chalmers, G. W. Luli, and W. R. Strohl
Glucose-stat, a glucose-controlled continuous culture”
Applied and Environmental Microbiology, vol. 57, no. 4, pp. 918-923, 1991. - G. L. Kleman, J. J. Chalmers, G. W. Luli, and W. R. Strohl
“A predictive and feedback control algorithm maintains a constant glucose concentration in fed-batch fermentations”
Applied and Environmental Microbiology, vol. 57, no. 4, pp. 910-917, 1991. - S. Goldblum, Y. K. Bae, W. F. Hink, and J. Chalmers
“Protective effect of methylcellulose and other polymers on insect cells subjected to laminar shear stress”
Biotechnology Progress, vol. 6, no. 5, pp. 383-390, 1990. - J. J. Chalmers, E. Kim, J. N. Telford, E. Y. Wong, W. C. Tacon, M. L. Shuler, and D. B. Wilson
“Effects of temperature on Escherichia coli overproducing beta-lactamase or human epidermal growth factor”
Applied and Environmental Microbiology, vol. 56, no. 1, pp. 104-111, 1990. - J. J. Chalmers, M. L. Shuler, and D. B. Wilson
“A simple membrane reactor to obtain high cell density and plasmid encoded protein production from Escherichia coli”
Biotechnology Techniques, vol. 4, pp. 73-78, 1990. - G. Georgiou, J. J. Chalmers, M. L. Shuler, and D. B. Wilson
“Continuous immobilized recombinant protein production from E. coli capable of selective protein excretion: a feasibility study”
Biotechnology Progress, vol. 1, no. 1, pp. 75-79, 1985. - F. H. Arnold, J. J. Chalmers, M. S. Saunders, M. S. Croughan, H. W. Blanch, and C. R. Wilke
"A rational approach to the scale-up of affinity chromatography"
ACS Publications, 1985.
1. Chalmers JJ, Melnik K, Lara-Velasco, OR, Tong X, Zborowski M, Moore LR, Farag S. Biological Cell Separator and Disposable Kit. US Patent 9,176,111; 2015
2. Sooryakumar, Ratnasingham; Henighan, Thomas Charles; Vieira, Gregory B.; Chalmers, Jeffrey J. Magnetic platforms for biomolecule trappings, manipulations, and sorting, Patent Number :US 08906691. December 9, 2014.
3. Sooryakumar, Ratnasingham; Sooryakumar, Dhriti; Vieira, Gregory; Chalmers, Jeffrey J. Mobile magnetic traps and platforms for micro/nano particle manipulation. U. S. Patent 6,623,984, March 19, 2013
4. Chalmers, J.J, Wu, W, Rathman, Compositions for reducing cell adhesion to bubbles. U.S. Patent 08440800 May 14, 2013.
5. Fleischman, A., Roy, S., Chalmers, J., Zborowski, M. MEMS-BASED Integrated Magnetic Particle Identification System. U. S. Patent 6,623,984, Sept. 2003.
6. Zborowski, M., Chalmers, J.J., Moore, L. System and device for determining particle characteristics. U.S. Patent 6,557,430, May 2003
7. Zborowski, M., Chalmers, J.J., Moore, L. System and Device for determining particle characteristics. U.S. Patent 6,412,359, October, 2002.
8. Zborowski M, Chalmers PS, Moore LR. Continuous particle and molecule separation with an annular flow channel, U.S. Patent 6,467,630, October 2002.
9. Zborowski, M., Chalmers, J.J. Moore, L. System and Device for Determining Particle Characteristics, U.S. Patent 6,082,205; 2000.
10. Zborowski, M., Chalmers, J.J. Moore, L. Fractional cell sorter, U.S. Patent 6,120,735, 2000.
11. Zborowski, M., Chalmers, J.J. Moore, L. Method for determining particle characteristics , U.S. Patent 6,142,025, 2000.
12. Zborowski, M., Chalmers, J.J. Moore, L., Method for magnetically separating cells into fractionated flow streams, U.S. Patent 5,968,820, 1999.
13. Zborowski, M., Chalmers, J.J. Moore, L. Method for Determining Particle Characteristics, U.S. Patent 5,974,901, 1999.